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 Now online (2)DADONCK recycle ...and 344 guests Last 5 registered Oplandisks nothingstar N_loop yipe foxtrotromeo Browse members... |
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Members 8025Messages 2614145 Today 17 Topics 127544 |
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gb2gbs faggot. |
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TRhats_Punping |
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A subclone of Chinese hamster ovary cells, R40F, has been selected for its unusually high resistance to lethal concentrations of Cd and Zn. Although there is a 33% loss in Cd resistance when R40F cells are cultured in the absence of exogenous metals, the Zn resistance remains unaltered. These cells are 80% tetraploid and demonstrate an increased capacity for metallothionein protein synthesis. When compared to wild type cells cultured in the absence of exogenous metals, R40F cells exposed to 200 M Cd for 48 h exhibited an approximate 200-fold increase in metallothionein-I (MT-I) protein. A 32P-labeled mouse MT-I cDNA was employed in solution hybridization studies to measure the level of MT-I mRNA in wild type and R40F cells. Cd (0.5 M) induces MT-I mRNA about 2.5- and 5-fold in wild type and resistant Chinese hamster ovary cells, respectively. When optimally induced, the resistant cells have about 80-fold more MT-I mRNA than the sensitive cells. Southern blot analysis of HincII-cleaved DNA indicates that the MT-I gene is amplified approximately 60- to 75-fold in R40F cells. |
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i hope you've got pics? |
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A/S/L? |
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